A simple, rapid, precise and accurate HPLC method for differentiating aspirin from the derivatives of Aspirin synthesized. The instrument used for carrying out HPLC was Shimadzu LCVP2010C integrated system equipped with quaternary gradient pump, Column utilized Kromasil C18 (180 X 4.6 mm), 5 µm, and the detection made possible using UV-Vis detector at a wavelength of 277 nm and the mobile phase employed was acetonitrile: methanol 60:40 (v/v). The Rt (Retention time) of aspirin was found to be 4.303 and that of derivatives as SR01- 2.543, SR02 -3.797, SR03-4.133, SR05-2.553, SR06-3.277, SR07-2.903. Proving the fact that the aspirin and its derivatives synthesized are pure.
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